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Cells were plated on 48-well plates, coated with anti-CD3 (1 g ml 1 ) and anti-CD28 (10 g ml ) antibodies, in RPMI medium or DMEM, supplemented with 10% FCS, penicillin, streptomycin, HEPES, glutamine, pyruvate, nonessential amino acids and -mercaptoethanol diluted with PBS (1:1), in the presence of IL-2 (10 ng ml 1 , Peprotech) and IL-12 (10 ng ml 1 , Peprotech) (T H 1 conditions), with or without amino acids

Hundered nanograms of rRNA-depleted RNA from each sample was fragmented into 200300 nts and used as a template for randomly primed PCR

As Gerry Melino (head of the Apoptosis and Cancer Laboratory, University of Leicester, UK) once said, without PCD, an 80-year-old person would have a 16 km long intestine and two tons of bone marrow and lymph nodes [23]

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