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Common cycling patterns include standard cycles of 8 weeks on followed by 4 weeks off, extended cycles of 12 weeks on followed by 6 weeks off for more aggressive protocols, and conservative cycles of 6 weeks on followed by 3 weeks off for those preferring a cautious approach

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The other three treatment groups were represented approximately equally in the largest cluster, with a maximum of one animal per treatment group outside the cluster

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On day 0, blood samples were collected via anesthesia-induced cardiac puncture and stored for plasma glycerol analysis