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Briefly, this vector was derived from a commercially available plasmid (VectorBuilder, Cat #VB010000-9782ymj) from which the f1 origin of replication and the lac operon system were removed to decrease the plasmid size

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Costimulation of murine osteoblasts with interferon-gamma and tumor necrosis factor-alpha induces apoptosis through downregulation of bcl-2 and release of cytochrome c from mitochondria