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Histology and adipocyte size and number analysis Paraffin embedding, slide sectioning and hematoxylin and eosin staining were performed by the Washington University Musculoskeletal Histology and Morphometry core

The freshly dissected kidneys were preserved at 80C for subsequent experiments

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doi: 10.3390/ijms232415479

2.3.3 Alkaloids Alkaloids, a significant class of naturally occurring nitrogen-containing organic compounds, most of which are derived from amino acids, are present in the coconut cotyledon [73, 79]