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U-87 MG cells were harvested from a human glioblastoma tumor, and although it is in their inherent nature to cluster when they are cultured in standard 2D culture, they adopt fibroblast-like morphology and only make small aggregates (Fig 1A)

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Biochanin A Attenuates Psoriasiform Inflammation by Regulating Nrf2/HO-1 Pathway Activation and Attenuating Inflammatory Signalling

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Consequences: Doubles apparent molecular weight on SEC May induce artificial dimerization of the passenger protein Can mask or distort protein-protein interactions For crystallization: GST dimer may dominate crystal contacts For some applications, the forced dimerization is actually useful (e.g., studying avidity effects)

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