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After incubation for 1 h, the DCFH-DA probe (10 M) was added and incubated in the dark for 30 min to detect ROS

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Because copper is both essential and potentially toxic in excess, the body maintains copper homeostasis within tight limits, and repeated systemic delivery of a copper-carrying molecule is, in effect, copper supplementation by an unusual route

GHK-Cu is compatible with most ingredients, including niacinamide and hyaluronic acid, but should not be combined with alpha hydroxy acids (AHAs) or strong acids in the same formula, as these may reduce efficacy

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Connecting to other gene-expression datasets Using GSEA, sets of genes reported to change with COPD-related phenotypes or with TGF treatment in other gene-expression studies were examined in a ranked list of genes ordered from most induced in severe emphysema to most repressed in severe emphysema by the t-statistic of the Lm coefficient in the linear mixed-effects model