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Materials and Methods Cell culture Zebrafish fibroblast cell line Pac 2 Zebrafish fibroblast cell line Pac 2 23,24 (CVCL_5853) was cultured in Leibovitzs L-15 medium containing phenol red (Sigma-Aldrich, Steinheim, Germany), supplemented with 15% fetal bovine serum (Gibco, Paisley, UK), 1% penicillin-streptomycin 100 U/mL (Gibco, Paisley, UK), 1 gentamicin 50 mg/mL (Lonza, Basel, Swiss)

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GLP-1 Semaglutide Semaglutide is a prescription treatment used in programs focused on weight management and appetite support for adults