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In mammals, the biosynthesis of melatonin starts with the conversion of tryptophan to l-5-hydroxytryptophan, which is converted to serotonin [5-hydroxytryptamine(5-HT)] by the aromatic l-amino acid decarboxylase (AADC)

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To confirm this hypothesis, we conducted cytotoxicity assays, and half-maximal inhibitory concentration (IC 50 ) values of the agents against ATC cells and normal thyroid cells were determined using the standard MTT assay (Fig