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Similarly, the variant of Cr FAP65AH2 did not show any interaction (Fig

10.1152/ajprenal.00083.2008 35 ElliottM

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50 L of solubilised lysate (input samplerepresenting total RNA) was taken prior to immune-precipation and spun overnight at 4C (same conditions as TRAP samples) before RNA-purification with TRAP samples

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On the other hand, these data may indicate that NMT1 indeed has the capacity to engage substrates even after extended elongation of the nascent peptide so that an entire domain has already emerged from the peptide exit tunnel