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Bound HA specific mouse antibodies were detected using an AP-conjugated anti-mouse IgG Fc specific antibody from goat (Sigma-Aldrich, A9544) (diluted 1:4000 in PBS/T/S) and visualized by addition of AP-substrate (10 g/mL in diethanolamine buffer)

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These nutrients assist in converting fat into energy and preventing fat buildup within the liver, creating a more efficient metabolic environment

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Figure 5E reveals that the fluorescent intensity was reduced after H 2 O 2 treatment, while incubation with targeting peptide RGD-GHK recovered COL-1 expression