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Malnutrition-related health outcomes in older adults with hip fractures: a systematic review and meta-analysis

urinaria extract, plays a crucial role in regulating the innate immune response of phagocytes, scavenging free radicals, acting as an antioxidant, and protecting the liver (Yuandani et al., 2013)

Carroll 2015

The supernatant was discarded and the beads were washed thrice with LSB-2 containing 150 mM NaCl which is termed LSB-3 (10 mM Tris pH 7.4, 1 mM EDTA, 10 mM -mercaptoethanol, 150 mM NaCl, and 1 mM PMSF) with or without IGEPAL CA-630 followed by incubation at 4 C/1 h

Hama N, Sunaga Y, Ochiai H, Kokaze A, Watanabe H, Kurosawa M, et al

Additionally, the purity and integrity of Trx1 were confirmed by electroblotting of samples resolved by 10% PAGE using semi-dry transfer on to a nitrocellulose membrane (22 m) and after blocking with 5% BSA in TBS for 30 min at RT, the membrane was immunoprobed with mouse monoclonal anti Trx1 antibody (Abcam Cat# ab16965, RRID:AB_443587