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Mass spectrometry analysis of UNC119B adducts after Lys-C digestion Samples of UNC119B (200 g protein per sample), either treated with compound 7 or vehicle DMSO, were processed by denaturation, reduction, alkylation, and digestion with rLys-C (Promega V1671), followed by desalting prior to mass spectrometry analysis

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J Exp Biol 201:12031209 Ganini D, Petrovich RM, Edwards LL, Mason RP (2015) Iron incorporation into MnSOD a (bacterial Mn-dependent superoxide dismutase) leads to the formation of a peroxidase/catalase implicated in oxidative damage to bacteria

After phase separation, the upper phase was collected and vacuum-dried