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[DOI] [PubMed] [Google Scholar] Alasil T, Wang K, Yu F, Field MG, Lee H, Baniasadi N, de Boer JF, Coleman AL, Chen TC, 2014

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For reverse transcription, first, a 10 L primer annealing mixture (7 L of modified RNA, 1 L of a 2 M reverse primer, and 2 L of 10 mM dNTPs) was prepared and incubated to anneal the primer