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Subsequently, pharmacologic inhibition of CD38 in LPS and IFN stimulated primary human astrocytes, phenocopied the murine studies and showed that the inhibition of NAD + hydrolysis in these cells, decreased chemokine signaling (72), supporting that astrocytic NAD + biology may play an important role in human neuroinflammation

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Next, we started to increase the temperature of the measurement chamber from 25 C to 50 C