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Mouse brains were collected, and tissue impregnation was performed by using a modified Golgi-Cox method according to the FD Rapid GolgiStain kit (FD Neurotechnologies, Columbia, MD)

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However, most if not all of the aforementioned agents are closely associated with adverse effects including prickling sensation, contact dermatitis, irritation, high toxicity and sensitivity (7-10)

10.1093/humrep/dey003 70

The authors found that a 14 day course of treatment with metronidazole given to healthy dogs, resulted in significant changes in microbiome composition, including severely decreased amounts of a key beneficial bacteria, Fusobacteria