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As hypothesized, a single dose of NAC (50100 mg/kg) completely inhibited the [GSH] decrease observed with naloxone and saline, and the protection persisted for at least 2 h

And not without good reason

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While, in the liver, NH 4 + is converted into urea, this cannot occur in the brain, where, instead, ammonia is incorporated, and thus detoxified, into the amide group of glutamine

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The thresholds for Iwp (high), Iwp (low) and Ip (low) were 200, 140, 80 for 4HNE, 200, 125, 75 for Cytochrome c, 200, 125, 75 for ferritin, 200, 115, 70 for ferroportin, 200, 150, 70 for GPX4 and 200, 135, 70 for NCOA4, respectively