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However, the utility of this testing has been the subject of heated debate (Quaas and Paulson, 2021

Blots were blocked for 1 h in 5% non-fat milk in phosphate-buffered saline (PBS) with 0.05% Tween-20, and membranes were probed with primary antibodies overnight and horseradish-peroxidase-conjugated secondary antibodies (Bio-Rad, Hercules, CA, USA)

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These include proteoglycans (PGs) [12], known as mineralization regulators, and N-linked glycoprotein, a small integrin-binding ligand that plays an important role in bone formation, among others [13]