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Equal amount of total protein from each lysate was incubated with 12.5 g of specified antibody bound to either protein A/G sepharose beads (Cytiva, 17096303/17061801), or streptavidin sepharose beads (Cytiva, 17511301), or S-protein agarose beads (Millipore, 69704) for 36 h at 4 C

Similarly, E GSH in rml1 roots has been shown to be far less negative than in wild-type plants (Meyer and Dick, 2010), but the exact value of cytosolic E GSH in this mutant has not been determined because the redox potential is close to the edge or even beyond the usable measuring range of roGFP2

Table 5 Functional analysis of bZIP transcription factor family in different plants

This effect, mediated through signal transducer and activator of transcription-6 (STAT-6), leads to increased oxidative stress and damage to mitochondria [20, 42]