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One way this has been overcome is through RP-HPLC methods, which retain reduced NAC as a stable, detectable molecule [46]Sana S, Rajani A, Sumedha N, Pravin P, Shripad N

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The absorbance at 405 nm was monitored at 20 C in a Synergy 2 microtiter plate reader, and the initial slope of the resulting curve was used as a measure of enzyme activity

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