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The study lasted 9 weeks, during which clinical signs associated with biotin deficiency and biochemical parameters, such as serum and tissue biotin levels (liver, kidney, brain, testes), and biotin-dependent enzyme activity of 3-methylcrotonyl CoA carboxylase (MCC), propionyl-CoA carboxylase (PCC), pyruvate carboxylase (PC) and acetyl-CoA carboxylase (ACC), and blood glucose levels, were evaluated