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coli To determine if immune cells in the blood from smokers were more or less responsive to an ex vivo challenge, we incubated freshly isolated whole blood samples for 7 h (to obtain levels of both early and late cytokines 21 ) in a 5% oxygen (to mimic in vivo conditions), humidified incubator at 37 C in the presence or absence of an immune cell challenge

A doctor also may use a pneumatic otoscope, which blows a puff of air into the ear canal, to check for fluid behind the eardrum

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