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Under physiological conditions, it is either remethylated to methionine or irreversibly metabolised through the transsulphuration pathway

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2017;12:e0189230

Under anesthesia, opened the abdominal cavity and exposed liver, inserted a catheter into the superior vena cava and perfused liver with buffer I (DHanks containing 180 mg/L EGTA and 80 mg/L NaOH) at a flow rate of 4.0 mL/min, followed by buffer II (DHanks containing 1 g/L collagenase IV and 444 mg/L CaCl2) at a flow rate of 2.0 mL/min