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Description

This system consists of pro-opiomelanocortin (POMC) and agouti-related peptide (AgRP) neurons in the arcuate nucleus of the hypothalamus (ARH), as well as downstream neurons expressing melanocortin receptors (MCRs) located in various brain regions

After three rinses in PBS, sections were incubated for 5 min with 4,6-diamidino-2-phenylindole (DAPI

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Metabotropic glutamate receptors are composed of mGluR18 subunits and have seven transmembrane segments that are connected to heterotrimeric G proteins

After pre-treatment, cells were stimulated with PMA and ionomycin at final concentrations of 10 ng/mL and 1 M, respectively, and incubated for an additional 4.5 h