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Scheibenbogen, Carmen (2020)

That creates the conditions tissue needs to actually heal [1]

doi: 10.1186/s13024-017-0218-4, 155 ZilkaO.ShahR.LiB.FriedmannA

Then, samples were centrifuged at 3660 g for 10 min and 150 L of the supernatant were transferred to a new microcentrifuge tube for dry vacuum

Depending on the experiment, a culture of 100 mL or of 2 L Luria-Bertani (LB) medium supplemented with 100 g/mL ampicillin (LB/Amp) was inoculated with 1/100 volume of an overnight culture and grown at 30 C to OD 600 0.4