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In vitro loss of IR impaired chondrogenic differentiation and reduced proteoglycan synthesis

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The protein was washed using a lysis buffer containing 0.2% Triton X and then buffer-exchanged to Protein Binding Buffer (20 mM HEPES pH 7.0, 150 mM KOAc, 2 mM Mg(OAc) 2 , 1 mM dithiothreitol, and 0.1% Tween) plus protease inhibitors

The Science TB-500 is a synthetic peptide derived from Thymosin Beta-4, a naturally occurring protein found in high concentrations in blood platelets, wound fluid, and other tissues

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