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Next is Berberine

Intention of the article is the collection of data to support the view that Wilson disease is a complex multifaceted and unique copper overload condition, where intracellular accumulation of toxic free copper, but not total copper, causes organ damage

Phalloidin staining The cochlea was fixed with 4% PFA in PBS at room temperature for 20 min, washed with 1 PBS, and incubated with 0.1% Triton X-100 at room temperature for 15 min