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Stable-isotope tracers including [U- 13 C]-glucose, [U- 13 C]-glutamine, [U- 13 C]-acetate, [U- 13 C]-serine, [U- 13 C]-cysteine, [U- 13 C]-glycine, and [U- 13 C]-pyruvate were purchased from Cambridge Isotope Laboratories (MA, USA)

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The procedure typically involves an intravenous infusion of a chelating agent, the most common of which is Ethylenediaminetetraacetic acid (EDTA)

Step 3: The Critical Injection This is where technique is everything

Fully anaerobic cells have three characteristics: a) very high level of HIF-1 expression and activation

Always use as directed with appropriate medication or as part of a compound preparation