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Nimgampalle et al., 2023

Bolotin, A

A.ResnerJ.LiottaA.KovcsR.HeinemannU

For first-strand cDNA synthesis and for real-time PCR analysis of relative miRNA expression, PerfeCTa universal primer and a PerfeCTa assay primer were used together with PerfeCTa SYBR Green SuperMix (95054

The disease mechanism centers on disrupted equilibrium between tissue breakdown and rebuilding within the disc, causing deterioration of the extracellular matrix, depletion of proteoglycans and hydration, decreased disc height, and compromised mechanical stability

Mesenchymal stem cells (MSCs) derived from the placenta, bone marrow, adipose, and other tissues have emerged as a promising regenerative therapy for PH over the last decade (Pullamsetti et al., 2014)