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Paula Williams: [LAUGH] All right

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L-CARNITINE 1500 Extreme MEGA CAPS/MEGA CAPSULES, a dietary supplement containing a concentrated dose of the purest, pharmaceutical form of L-carnitine tritrate, with the optimal degree of micronisation.Each capsule due to the use of MEGA CAPS/MEGA CAPSULES technology contains as much as 1000 mg of pure L-carnitine

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Skill: Deducing the DNA base sequence for the mRNA strand mRNA DNA mRNA is a complementary copy of a DNA segment (gene) and consequently can be used to deduce the gene sequence For converting a sequence from mRNA to the original DNA code, apply the rules of complementary base pairing: Cytosine (C) is replaced with Guanine (G) and vice versa Uracil (U) is replaced by Adenine (A) Adenine (A) is replaced by Thymine (T) Example: (mRNA) AUG CCA GUG ACU UCA GGG ACG AAU GAC UUA Answer: (DNA) TAC GGT CAC TGA AGT CCC TGC TTA CTG AAT Skill: Use a table of mRNA codons and their corresponding amino acids to deduce the sequence of amino acids coded by a short mRNA strand of known base sequence mRNA Polypeptide In order to translate an mRNA sequence into a polypeptide chain, it is important to establish the correct reading frame The mRNA transcript is organised into triplets of bases called codons, and as such three different reading frames exists An open reading frame starts with AUG and will continue in triplets to a termination codon A blocked reading frame may be frequently interrupted by termination codons Once the start codon (AUG) has been located and reading frame established, the corresponding amino acid sequence can be deduced using the genetic code Example: (mRNA) GUAUGCACGUGACUUUCCUCAUGAGCUGAU Answer: (codons) GU AUG CAC GUG ACU UUC CUC AUG AGC UGA U Answer: (amino acid) Met His Val Thr Phe Leu Met Ser STOP The Genetic Code (Grid)

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Tissue Lysis and Protein Extraction Tissue powder was thawed by adding Lysis Buffer (5% SDS, 50 mM Tris pH 6.5-7.0, 25 mM TCEP, 20 mM Iodoacetamide (IAC)) at a volume of 130 L per 20 mg of tissue powder

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