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Most chronic diseases are related to oxidative stress arising from the affected tissue losing the capacity to maintain glutathione at adequate healthy levels

Nature 365 , 797805 (1993)

Annu Rev Microbiol 54:439461 Choi JH, Lou W, Vancura A (1998) A novel membrane-bound glutathione S -transferase functions in the stationary phase of the yeast Saccharomyces cerevisiae

hPD-L1 MC38 cells were cultivated in Dulbeccos Modified Eagle Medium containing 10% (v/v) fetal bovine serum (FBS), 50 g/mL hygromycin B, and antibiotics (100 U/mL penicillin and 100 g/mL streptomycin)