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Plating densities were as follows: 25,000 cells/well in 100 l/well for 18-well chamber slides (Ibidi, 81817) and poly-D-lysine coated 96-well plates (Corning, 354461), or 150,000 cells/well in 500 l/well for poly-D-lysine coated 24-well plates (Fisher, 08-774-124)

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Franz AP, Bolat GU, Bolat H, Matijasevich A, Santos IS, Silveira RC, et al

Why Layering Matters with Copper Peptides Copper peptides perform best when layered with hydrating, barrier-supporting ingredients and when kept away from strong acids or actives that can reduce their effectiveness

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