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Photosynthesis of cucumber plants was depressed under the HT treatment based on the light and CO 2 response curves, which could be due to the reduction of chlorophyll content and carotenoid content at high temperatures that impaired the biosynthesis of photosynthetic pigments 33

Always take up more of the gradient medium than is required for the step as it is more accurate to empty the syringe to a graduation mark than to try to empty it completely