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Always reconstitute with fresh, pharmaceutical-grade bacteriostatic or sterile water, add it slowly down the inner wall of the vial, and swirl gently instead of shaking

For cell viability assay, 8505 C and SNU-80 cells (1 10 4 and 5 10 3 cells/well) grown in 96-well plates were treated with glutamine-free medium or reagents for the indicated times

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The combination of ATRA and conventional chemotherapy selectively improved the survival of AML patients with NPM1 mutation in the absence of FLT3-ITD [52]