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Binding dCas9 to the transcription start site (TSS) of a gene can block the start of transcription, thereby inhibiting gene expression (Figure 1C)

10.1136/gut.50.2.241 131

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This limitation is particularly relevant in rice, where recombination rates vary markedly along chromosomes, such that any single kb cM 1 conversion represents an approximation rather than a precise local measure [ 88