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Supernatant containing the protein portion was added with sample buffer (0.125 M Tris [pH 6.8], 10% [v/v] glycerol, 0.02% SDS, and 1 mM dithiothreitol [DTT]) and subsequently heated at 95 C for 5 min

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Bava S, Theilmann RJ, Sach M, May SJ, Frank LR, Hesselink JR, Vu D, Trauner DA (2010) Developmental changes in cerebral white matter microstructure in a disorder of lysosomal storage

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These observations suggest the critical roles of estrogen in normal metabolism function for glucose metabolism and energy homeostasis and that estrogen-deprivation induced systemic inflammation which is associated with increased glycolysis, increased lactate production and reduced TCA cycle activity and these effects were revered upon estrogen supplementation

This is generally done over 5 weeks, although it may need to be renewed once depending on your metabolism