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Turner and Renegar, 2017)

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The proton-motive force created by the ETC is also used for many additional mitochondrial processes, especially those related with transport across the IMM ( Although the primary function of mitochondria is to generate ATP as an energy molecule required for countless cell functions, a small fraction of electrons from the ETC are transferred directly to O 2 , resulting in the generation of the superoxide anion, which can give rise to other ROS as well as reactive nitrogen species (RNS)

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1 g of peptides were separated on a 50 cm length 75 m internal diameter (ID) C18 Pepmap Acclaim column (Thermo Fisher) using a linear 90-minute acetonitrile gradient