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Aconitase activity, a Krebs cycle enzyme highly sensitive to superoxide, was determined by the method described by Morrison (1954), using a medium containing 50 mM KH 2 PO 4 , 10% triton X-100, 0.07 mM sodium citrate, 1.3 mM manganese chloride

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however, many clinical trials fail, and patients experience an unsatisfactory treatment response, likely owing to IC/BPS phenotype heterogeneity and the use of non-targeted interventions

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Some research findings show that high-protein intake can cause oxidative stress, which may lead to increased risk of chronic disease, including cancer, while other findings did not show any correlation between high-protein diets and long-term increase in ROS levels (271, 274, 275)