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Phytochemistry 59 (5), 537542

The residues selenium-cysteine (U) or cysteine (C) at the active site, together with glutamine (Q) and tryptophan (W), forms the signature catalytic triad of the glutathione peroxidase family 30

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When GSTP was silenced, the levels of ROS had reached 3.7-fold over that of control cell (Fig

Furthermore, it is worth highlighting that there are important differences between the studies evaluated, such as the supplementation protocol (dose, supplementation with free glutamine or associated with other nutrients, etc.), the exercise protocol (short-term exercise and aerobic, long-term exercise and endurance or intermittent), characteristics of the volunteers (gender, age, physical activity level, etc.), among others, which might partially explain the controversial results obtained

Membranes were sedimented by ultracentrifugation at 150,000 g at 4 C for 1 h, and resuspended in 16 mL of solubilized buffer (1%(w/v) DDM, 150 mM NaCl, 10%(v/v) glycerol, 1 mg mL 1 iodoacetamide, 0.2 mM TCEP, 0.1 protease inhibitor cocktail, 50 mM Tris-HCl pH 8.0)