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The proteins were eluted using a 0400 mM NaCl gradient, concentrated by ultrafiltration and stored in 30 mM Tris-HCl pH 8.0, 200 mM NaCl buffer

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There have been negative effects observed as well, suggesting that DPI (and other related structures) act as pro-oxidants by decreasing GSH (Riganti et al., 2004)

The Roles of Serotonin in Neuropsychiatric Disorders