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The catalytic core of class II ARS is comprised of seven anti-parallel sheets flanked by -helices and harbors three conserved motifs [7, 8, 18]

Upregulation of p16 leads to the hypophosphorylation of the pRb protein and induction of senescence 11,40

It is very likely that life-prolonging effect of spermidine goes beyond a simple increase in the number of days lived, and that relies on retardation of the manifestation of major age-associated diseases , like cardiac and arterial aging 106,114 , colon cancer 115 , neurodegenerative processes 116,117 , and its anti-inflammatory effects 112

Carnosine is the safer, more accessible, and better-validated option with extensive human data, oral availability, and multi-pathway protective effects against oxidative stress and glycation

The hPrdx6 concentration used for the CD measurements was 0.4 g/l (16 M)

The ROS generated from aerobic respiration caused a cumulative oxidative damage in macromolecules, including lipids, DNA, and proteins, which subsequently lead to cells death (Scheibye-Knudsen et al., 2015), and affect the healthspan of numerous principal organ systems (Dai et al., 2014)