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Glo-1 is the rate-limiting enzyme for the system and is dependent on GSH ( , leading to ferroptosis

Statistical analysis All experiments contained at least three independent replicates

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Cagri is N-terminally acylated with a Glu linker and a C20 fatty diacid moiety

Thus, unlike previous in vitro biochemical studies or metabolic engineering efforts, which had excluded arGSTs, we set out to confirm our hypothesis by performing coupled enzymatic reactions with 3,4- cis -leucocyanidin ( 6 ) combining clarified Escherichia coli cell lysates expressing At LDOX and the well-studied arGST Ph AN9 from petunia