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Many cells can secrete TC, which combines with free B12 in the cells of the ileum to form holotranscobalamin (holoTC), which enters the portal circulation and is responsible for delivering the vitamin to the cells, as it transports the functional fraction that can be absorbed by the tissues and is rapidly released into them by endocytosis (the process of incorporating molecules into cells) through their specific receptors

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For experiments, CD8 + T cells were seeded at a density of 5 10 5 cells per ml medium

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