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Description

Diab Obes

Nerve irritation

Int J Mol Sci

doi: 10.1016/j.biocel.2010.11.008 51 ChowFOzolsENikolic-PatersonDJAtkinsRCTeschGH

Pharmaceutical-grade peptides undergo rigorous purity testing, sterility verification, and quality control measures required for human therapeutic use

Roomtemperature lysis buffer (150 mmol NaCl, 25 mmol Tris, 0.1% NP40, and complete protease INHIBITOR) with 50 g of GSTtagged thiophosphorylated Arppt19 or Nipp1 central domain was used to resuspend cell pellets and immediately transferred to a thermomixer at 600 rpm for 5 min