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The final amplification cycle was followed by a 7 minutes elongation at 65 C

Zenobi PD, Graf S, Ursprung H, Froesch ER

Konopleva M, Marini F, Leysath C, Champlin R, Andreeff M , Korbling M

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Physiol Rev 81(1):209237 Takeda K, Machida M, Kohara A, Omi N, Takemasa T (2011) Effects of citrulline supplementation on fatigue and exercise performance in mice