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NAD+ also helps protect your cells from oxidative damage

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SIRF U2OS cells growing on autoclaved coverslips were incubated with 25 M EdU for 10 min, washed twice with ice-cold PBS, pre-extracted with cold 0,2% (v/v) Triton X-100 in PBS for 5 min on ice and fixed with 4% (v/v) formaldehyde solution for 15 min