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FIGURE 9 3.2.7 The selenocysteine adventure The development of the previously mentioned tRNA Sec UTu represents a significant achievement as it allows SECIS-independent selenocysteine incorporation at the amber codon ( Sec UTu by designing a new tRNA, tRNA Sec UTuX (Miller et al., 2015) based on the co-crystal structure of Aquifex aeolicus SelA and Thermus tengcongenisis tRNA Sec ( Sec UTuX not only enhanced SelA-mediated conversion compared to the original tRNA Sec UTu but also improved serine charging efficiency by SerRS, resulting in an increase in the yield of selenoprotein synthesis

2 [31]) of which 17 papers reporting 20 cases were included in the detailed analysis

Proteomics 74 , 765773 (2011)

[DOI] [PMC free article] [PubMed] [Google Scholar] 89.Verdoia M, Nardin M, Gioscia R, Saghir Afifeh AM, Viglione F, Negro F, Marcolongo M, De Luca G

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This pathway starts with delivering iron and sulfur ions to scaffold protein ISCU2