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Cellular mechanisms of redox cell signalling: role of cysteine modification in controlling antioxidant defenses in response to electrophilic lipid oxidation products

Prevalence and characteristics of autism spectrum disorder among children aged 8 years - autism and developmental disabilities monitoring network, 11 sites, united states, 2018

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10.3389/fimmu.2023.1228928 113 Rodrguez-EgurenA.Gmez-lvarezM.Francs-HerreroE.RomeuM.FerreroH.SeliE.et al (2022)

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On the day of the assay, cells were washed twice with MAS solution (mannitol 220 mM, sucrose 70 mM, KH 2 PO 4 10 mM, MgCl 2 5 mM, HEPES 2 mM, EGTA 1 mM, brought to pH 7.2 using KOH) and then incubated with assay media (MAS solution supplemented with 0.2% BSA-free fatty acids, 4 mM ADP, 10 mM pyruvate, 0.5 mM malate, and 1 nM Plasma Membrane Permeabilizer (Seahorse XF Plasma Membrane Permeabilizer, PMP, cat.no