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Tissue samples were sonicated in PBS supplemented with protease and phosphatase inhibitors (Complete protease inhibitor cocktail, Roche)

On the one hand, the development of stable GLP1R agonists and DPP-4 inhibitors has overcome the limitations imposed by the short half-life of endogenous GLP-1, providing sustained action and clinically validated efficacy in improving glycemic control and reducing cardiovascular risk

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Miquilena-Colina ME, Lima-Cabello E, Sanchez-Campos S, Garcia-Mediavilla MV, Fernandez-Bermejo M, Lozano-Rodriguez T, Vargas-Castrillon J, Buque X, Ochoa B, Aspichueta P, Gonzalez-Gallego J, Garcia-Monzon C (2011) Hepatic fatty acid translocase CD36 upregulation is associated with insulin resistance, hyperinsulinaemia and increased steatosis in non-alcoholic steatohepatitis and chronic hepatitis C

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10.1155/2011/863734 132 MishraV.SorenA