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The reduction introduces a hydrophilic group in the drug molecule or unmasks the already present hydrophilic group

The mixture is then passed through a fresh glutathione resin column to remove the cleaved GST tag, allowing the purified target protein to be collected in the flow-through

J., Fiebig, A

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Despite the different structures and functions of molybdenum-dependent enzymes, there are important similarities, which we highlight here