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Then, 4.88 mL of simulated intestinal fluids containing 16 U/mL pancreatin (lot number SLCM8903, trypsin activity = 8.9 U/mg) and 3.1 mM bovine bile (lot number SLBV1780, bile salts concentration = 440 g/mol) were added

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The membrane was incubated with rabbit anti-Glutathione Peroxidase 4/GPX4 antigen affinity purified polyclonal antibody (Catalog # A02059-1) at 0.5 g/mL overnight at 4C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT

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Cell-Free Synthesis and Multifold Screening of Candida Antarctica Lipase B (CalB) Variants after Combinatorial Mutagenesis of Hot Spots